FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8583spike-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8583spike-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7134442
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT711490.997260893003265TruSeq Adapter, Index 20 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG90350.038.3633174-75
GCCGTCT94100.037.6835550-51
TGCCGTC96750.036.8942850-51
GAGCACA196350.036.4308249
GTATGCC102400.034.9733546-47
ATGCCGT102950.034.85498448-49
TATCTCG99650.034.8080440-41
CGTATGC103350.034.58366446-47
TATGCCG104200.034.41430748-49
AGAGCAC208500.034.3980458
ACGTGGC102750.034.12380632-33
GGCCTTA103450.033.734636-37
CTCGTAT105750.033.73211744-45
CGTGGCC103850.033.67184432-33
CACGTGG104650.033.43666530-31
AAAGGGG104200.033.4220272-73
AAGGGGG95950.033.30781674-75
CCGTCTT107100.033.2630452-53
TCGTATG107750.033.04057344-45
ATCTCGT108850.032.55669842-43
Produced by FastQC (version 0.11.2)