FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8583spike-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8583spike-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6523069
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT1924442.9502064135761863TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA321550.061.3006029
AGAGCAC332750.059.350288
CGGAAGA372850.053.2762764
TCGGAAG374000.053.1753163
GAAGAGC373300.053.136496
AAGAGCA381000.052.0068367
GATCGGA386400.051.0786861
ATCGGAA393000.050.701762
GGAAGAG395350.050.2680245
AGGGGGG219250.043.8903674-75
GCCGTCT222200.043.41341450-51
TGCCGTC225450.043.3712950-51
GTATGCC227950.042.8131546-47
CGTATGC231300.042.33530446-47
TATCTCG227650.042.28150640-41
ATGCCGT230250.042.27321648-49
TATGCCG231600.042.26017848-49
CCGTATC231700.042.1719138-39
CTCGTAT231000.042.0037244-45
AAAGGGG229950.041.79696372-73
Produced by FastQC (version 0.11.2)