FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8583-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8583-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6160211
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC504900.8196147826754636TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG68750.037.46228472-73
GCCGTCT73250.035.99495348-49
TGCCGTC74600.035.37507248-49
AAGGGGG72700.034.8773572-73
GATCTCG74450.034.50026738-39
CGATCTC74100.034.4095138-39
GTATGCC79450.033.39307844-45
CACGATC76650.033.14213636-37
CGTATGC80750.032.59356744-45
TATGCCG82000.032.29731846-47
ATGCCGT82400.032.2260946-47
CTCGTAT81650.031.88918742-43
AAAGGGG82950.031.19085770-71
GAGCACA171300.031.058149
ATCTCGT82950.030.82282340-41
TCGTATG86450.030.7709842-43
CCGTCTT87250.030.35392850-51
TCTCGTA85950.030.12975740-41
TCACGAT86450.030.06471434-35
CATCACG89300.029.65747332-33
Produced by FastQC (version 0.11.2)