FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8583-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8583-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5238204
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC1828723.4911202389215843TruSeq Adapter, Index 5 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGC133650.255144702268182TruSeq Adapter, Index 5 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA326500.063.7109959
AGAGCAC335500.062.099978
TCGGAAG369250.056.7229083
CGGAAGA369100.056.707754
AAGAGCA374000.055.883267
GAAGAGC373900.055.841446
GATCGGA375000.055.3147281
ATCGGAA381950.054.8871352
GGAAGAG385650.054.2010155
GCCGTCT229400.043.85387448-49
AGGGGGG228050.043.77343472-73
AAGGGGG232450.043.1874872-73
CGTATGC237500.043.1794744-45
TGCCGTC235850.043.16271248-49
GTATGCC238800.042.97392744-45
TATGCCG239400.042.79751246-47
ATGCCGT238950.042.7010946-47
GATCTCG217450.042.49230638-39
TCGTATG240100.042.32039642-43
AAAGGGG236150.042.30184670-71
Produced by FastQC (version 0.11.2)