FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8151spike-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8151spike-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6347240
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGTAATCTCGTAT227140.3578563281048141TruSeq Adapter, Index 22 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG36850.031.18332574-75
AAGGGGG41400.026.05333774-75
GCCGTCT45100.025.94799850-51
GTATGCC49000.024.26640746-47
TGCCGTC49200.024.1250-51
CGTACGT49950.023.66542634-35
CCGTACG49750.023.66534232-33
CGTATGC50850.023.47598346-47
TATGCCG51000.023.45301448-49
CTCGTAT50500.023.45255744-45
AAAGGGG49900.022.9339872-73
ATCTCGT52950.022.27917342-43
ATGCCGT52600.022.2481948-49
TCTCGTA54950.021.46828542-43
CCGTCTT55500.021.25503352-53
TCGTATG56000.021.23310544-45
GTACGTA56450.020.85718234-35
CGTAATC56800.020.81140938-39
AATCTCG60150.019.534240-41
ACCGTAC60950.019.3552232-33
Produced by FastQC (version 0.11.2)