FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8151spike-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8151spike-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6650254
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT4652606.996123757077549TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA651900.074.798289
AGAGCAC663700.073.560488
GAAGAGC708650.069.01896
CGGAAGA712600.068.735284
TCGGAAG713100.068.6282653
AAGAGCA714050.068.472227
GATCGGA724050.067.429871
GGAAGAG728550.067.165945
ATCGGAA730600.067.146822
AGGGGGG521700.045.09770674-75
GTCCCGA526000.044.97096336-37
CGTCCCG534900.044.59174334-35
GTATGCC543650.044.55201346-47
AAAGGGG526350.044.49392372-73
GCCGTCT543150.044.45025350-51
CCCGATC524450.044.4451938-39
CCGATCT522800.044.4281438-39
TCCCGAT525900.044.3762736-37
TGCCGTC547100.044.30543550-51
CGTATGC548050.044.24149346-47
Produced by FastQC (version 0.11.2)