FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8115spike-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8115spike-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6159543
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTAT608940.9886123045167474TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG79050.038.8828474-75
GCCGTCT86100.036.57242250-51
GTATGCC89400.035.2487246-47
TGCCGTC89950.034.95481550-51
TATGCCG90700.034.84713448-49
TATCTCG90650.034.5307640-41
AAGGGGG80650.034.29447674-75
ATGCCGT93150.033.85491648-49
CGTATGC93400.033.6888246-47
AAAGGGG90850.033.651572-73
CTCGTAT94500.033.42100544-45
CACGAGT93600.033.36672230-31
CGAGTGG93900.033.18517732-33
ACGAGTG95300.032.82096532-33
GAGCACA194500.032.548439
CCGTCTT97400.032.45005452-53
TCTCGTA99650.031.67122542-43
ATCTCGT100150.031.51310742-43
TCGTATG100150.031.51208344-45
AGTCACG101200.031.11723728-29
Produced by FastQC (version 0.11.2)