FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8115spike-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8115spike-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5651566
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTAT934281.6531347240747076TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACA227750.042.7785769
AGGGGGG116250.040.772674-75
AGAGCAC241850.040.3039938
GTATGCC121150.039.99637646-47
AAAGGGG119050.039.81364472-73
CATCTCG121650.039.34975440-41
GCCGTCT124150.038.9541850-51
ATGCCGT124550.038.9422848-49
TGCCGTC124750.038.63496850-51
CTCGTAT125700.038.11863744-45
CCGCACA124350.038.09883536-37
CGTATGC126750.038.0438846-47
ACGTCCG128350.037.25887732-33
TCGTATG129650.037.17479344-45
CGCACAT129850.036.77466236-37
CGTCCGC130750.036.7187532-33
CCGTCTT132400.036.42040652-53
ATCTCGT132050.036.16166342-43
GCACATC131400.036.1083738-39
GTCCGCA131950.036.1003434-35
Produced by FastQC (version 0.11.2)