FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8104spike-inp_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8104spike-inp_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6293112
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC203510.3233853139750254TruSeq Adapter, Index 10 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG36950.028.74577372-73
AAGGGGG41150.026.611372-73
TGCCGTC38450.026.4631548-49
GCCGTCT37750.026.2691248-49
TATCTCG44600.023.92244338-39
GTATGCC45600.023.03523844-45
TATGCCG45200.023.03113446-47
CGTATGC46900.022.64725944-45
ATGCCGT45300.022.56529846-47
CTCGTAT47500.021.66909842-43
CCGTCTT49900.020.061350-51
ATCTCGT52850.019.9206640-41
AAAGGGG53400.019.8905770-71
TCTCGTA52550.019.81078740-41
TCGTATG52800.019.71651542-43
CACTAGC58250.019.24382830-31
GCTTATC60400.018.36494336-37
ACTAGCT62350.018.2046132-33
TAGCTTA62300.018.0689134-35
CTGCTTG54650.017.84464656-57
Produced by FastQC (version 0.11.2)