FastQCFastQC Report
Wed 15 Nov 2017
HVJH5AFXX_n01_ah8104spike-chip_11082017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJH5AFXX_n01_ah8104spike-chip_11082017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8275537
Sequences flagged as poor quality0
Sequence length100
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGC1961522.370263101959426TruSeq Adapter, Index 12 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG219300.043.17281372-73
GAGCACA459700.042.8269969
TGCCGTC233700.041.7896348-49
GCCGTCT233300.041.46845648-49
AAGGGGG229550.041.3371772-73
AGAGCAC479750.041.125318
ATGCCGT239800.040.53060546-47
AAAGGGG234150.040.5050170-71
GTATGCC241750.040.310644-45
TATGCCG243750.040.24014746-47
CGTATGC244500.040.08787544-45
CCGTCTT245650.039.69931850-51
CTCGTAT243200.039.63688742-43
TCGTATG251100.038.65189742-43
ATCTCGT251500.038.02978540-41
CTGCTTG253250.037.96048756-57
TCTCGTA250600.037.92255440-41
TGTAATC263700.036.3333936-37
TGCTTGA268550.036.2002958-59
CGTCTTC268450.036.13499550-51
Produced by FastQC (version 0.11.2)