FastQCFastQC Report
Thu 19 Apr 2018
HVJG3BGX5_n02_4_cut16h-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJG3BGX5_n02_4_cut16h-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89022601
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2373260.26659072789841315No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA1024450.015.44159869
AGTGTAG803800.015.00178369
CGAGTGT1237700.014.80208269
ACGAGTG1552050.012.62437369
CTCGGTG148550.012.4954969
GTGTAGA716500.011.6194369
GACGAGT1805900.011.25483269
ATCTCGG225100.010.52982868
TCTCGGT223800.010.40608469
CGACGAG2097250.010.25558169
GTATAGG170900.010.1376731
GTATTAG469750.09.8670171
GTATAGA272950.09.3820441
GTATAAG371100.09.2442591
GTATATA484200.09.2232961
GTATATG473450.09.0901071
CCGACGA2426650.08.85769968
GTGTAAG191150.08.8470491
GTCTAGG74300.08.6397431
GATCTCG280050.08.611629569
Produced by FastQC (version 0.11.2)