FastQCFastQC Report
Thu 19 Apr 2018
HVJG3BGX5_n02_3_cut16h-2000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJG3BGX5_n02_3_cut16h-2000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95121113
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32888703.457560468200157No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG448400.025.45313669
TCTCGGT688700.020.34938269
ATCTCGG843050.016.89370268
GATCTCG1034600.013.81596267
TCGGTGG318650.013.69676569
AGTGTAG2845600.013.67170369
GAGTGTA3484200.013.08496769
GTGTAGA2334100.012.49490269
CGAGTGT4151650.012.38661669
AGATCTC1207100.011.87301466
GTAGATC1597950.010.64233869
ACGAGTG4866050.010.64112869
TGTAGAT2081350.010.62064769
TAGATCT1508500.09.58070265
GACGAGT5579800.09.29658168
CCGTGAG135500.08.93654259
GTATTAG343200.08.64791
TATACCG238500.08.6210015
GTATAGA268250.08.5682871
TACCGTG158800.08.2553417
Produced by FastQC (version 0.11.2)