FastQCFastQC Report
Thu 19 Apr 2018
HVJG3BGX5_n02_2_cut16h-2000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJG3BGX5_n02_2_cut16h-2000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87809953
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1836490.20914371745535498No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTGTA1032200.015.01116669
AGTGTAG808650.014.85178469
CGAGTGT1229850.014.56524469
CTCGGTG147550.013.49185969
ACGAGTG1538350.012.47863869
GTGTAGA712550.012.18717569
ATCTCGG221450.012.0741768
TCTCGGT224700.011.82286869
GACGAGT1771950.010.86470569
GTATAGG172100.010.3877591
GTATAGA282750.010.1309111
GATCTCG274500.09.94180367
CGACGAG2043250.09.86619269
GTATTAG484250.09.6498821
GTATAAG342650.09.6491211
GTATATA503150.09.2462451
GTATATG489200.08.9525791
GTAGATC471800.08.78254369
CCGACGA2350050.08.69560669
GTATAAA755850.08.6525991
Produced by FastQC (version 0.11.2)