FastQCFastQC Report
Thu 19 Apr 2018
HVJG3BGX5_n02_1_cut16h-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJG3BGX5_n02_1_cut16h-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99049544
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3925250.39629157707177326No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTG204850.019.5878169
TCTCGGT320800.015.75597969
AGTGTAG1426900.015.63692969
GAGTGTA1824250.015.19082969
ATCTCGG354100.014.41055368
CGAGTGT2202950.014.17379269
GTGTAGA1187400.013.33117869
ACGAGTG2721000.012.39580569
GATCTCG432000.011.90778567
GACGAGT3152300.010.68548268
GTAGATC771450.010.0538169
GTATAGG191950.09.7635071
AGATCTC537300.09.58049566
TGTAGAT1196100.09.42663669
CGACGAG3618800.09.38436869
GTATAGA303350.08.8062511
CCGACGA4143250.08.52875769
TAGATCT789000.08.470221569
GTATATA535450.08.3214961
GTATAAG403750.08.2918851
Produced by FastQC (version 0.11.2)