FastQCFastQC Report
Thu 19 Apr 2018
HVJG3BGX5_n01_4_cut16h-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJG3BGX5_n01_4_cut16h-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89022601
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG322150.020.86995569
GAGGATC478050.017.14418833
AGGATCG330600.015.58425534
GAGAGGA603350.014.51532531
GTATGCC215550.013.78488142
AGAGAGG646250.013.56736230
GACCAGA1178850.013.44605369
GATCGCG192750.013.33871336
GATCTCG185750.013.17253836
CAGAGAG664850.013.07838829
ACCAGAG942950.012.79307169
AGACCAG1456200.012.68309269
GGATCGC207450.012.40972835
GATCGGG224050.012.16841936
TATGCCG242850.011.77919243
GGATCGG227450.011.77369335
TGCCGTC180050.011.74727545
ATCGGGG166700.011.696774537
GAGACCA1732800.011.63003369
CGTATGC232550.011.33781941
Produced by FastQC (version 0.11.2)