HVJG3BGX5_n01_3_cut16h-2000.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HVJG3BGX5_n01_3_cut16h-2000.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	95121113
Sequences flagged as poor quality	0
Sequence length	75
%GC	41

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG	1334156	1.4025866160754448	RNA PCR Primer, Index 39 (96% over 30bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGGG	514894	0.5413035905078192	RNA PCR Primer, Index 39 (96% over 30bp)
TCTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAGGGGG	166015	0.17453012771202542	Illumina PCR Primer Index 11 (96% over 29bp)
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAATGGGGG	133709	0.1405671104794579	RNA PCR Primer, Index 39 (96% over 30bp)

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
AGGGGGG	80265	55.389767	69
GCCGTCT	334345	53.175556	46
CGTATGC	343615	53.1588	41
GTATGCC	345745	52.825928	42
TGCCGTC	343430	52.527138	45
CTCGTAT	343180	52.389908	39
TCGTATG	347180	52.32531	40
TATGCCG	348685	52.29284	43
ATGCCGT	347655	52.122417	44
TCTCGTA	338880	51.77578	38
CCGTCTT	343795	51.713787	47
CGTCTTC	349765	50.512142	48
ATCTCGT	345170	50.287056	37
CTGCTTG	350690	49.57558	54
CATCTCG	354375	48.98486	36
CTTCTGC	364155	48.020332	51
TGCTTGA	366070	47.65464	55
TCTGCTT	365840	47.592617	53
GTCTTCT	370495	47.380314	49
GCTTGAA	372930	47.04778	56