FastQCFastQC Report
Thu 19 Apr 2018
HVJG3BGX5_n01_2_cut16h-2000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJG3BGX5_n01_2_cut16h-2000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87809953
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT222100.025.43045846
TGCCGTC245950.023.10547845
GTATGCC271600.021.49893642
GATCTCG261800.020.81432336
CGTATGC284400.020.38591841
TATGCCG289650.019.84702343
ATGCCGT325300.018.0744144
CCGTCTT320150.017.79272547
CTCGTAT321300.017.60432239
CGTCTTC336150.017.0380948
TCTCGTA333000.016.66454138
TCGTATG368850.015.91507140
ATCTCGT352900.015.75410537
GACTAGG1061100.014.82111469
ACTAGGC841750.014.47455169
AGACTAG1346050.013.63639569
AAGGGGG289100.013.39982769
CTAGGCA653950.013.05036569
GTCTTCT443200.013.00053349
TGATCTC441750.012.72554935
Produced by FastQC (version 0.11.2)