FastQCFastQC Report
Thu 19 Apr 2018
HVJG3BGX5_n01_1_cut16h-1000.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJG3BGX5_n01_1_cut16h-1000.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99049544
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGGGGG1281160.1293453708378506RNA PCR Primer, Index 27 (96% over 30bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT416200.038.57346
TGCCGTC445000.037.14021345
GTATGCC480450.035.46990242
CGTATGC498100.034.0538941
TATGCCG506700.033.4646743
CTCGTAT538150.031.41696739
TATCTCG537550.031.29145236
CCGTCTT519750.031.0467847
ATGCCGT541050.030.9750444
TCTCGTA546850.030.53865838
CGTCTTC552800.029.09674548
ATCTCGT578700.029.07840537
TCGTATG592950.028.68824640
CTATCTC659700.025.56460635
AGGGGGG169400.025.43397369
AAGGGGG464400.024.56445969
CCTATCT697800.024.25743734
GTCTTCT690600.023.19093749
TCCTATC817250.021.17625433
CTGCTTG786700.019.9975754
Produced by FastQC (version 0.11.2)