FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40377441
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA741100.032.254224
ATCGGAA759900.032.0620462
TCGGAAG750650.031.9299093
AGAGCAC710750.031.8952228
GATCGGA775700.031.4766581
GAGCACA851300.026.7352459
CGTCTGA439750.024.36722216-17
ACGTCTG446850.023.78849414-15
ACACGTC462450.023.40171612-13
CGTTCGA14350.023.34577432-33
CACGTCT464350.023.3008514-15
GAAGAGC1022350.023.0978556
AAGAGCA1013400.023.0723427
CACACGT508100.021.78497112-13
GGAAGAG1152600.020.8212415
GCACACG531750.020.6420310-11
GAACTCC524400.019.91454720-21
TCACGTA99000.019.5698230-31
CTGAACT556950.018.91631118-19
CGTACGA38750.018.39461132-33
Produced by FastQC (version 0.11.5)