FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb378.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb378.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31595516
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT59350.012.97351
CCCGTAT42400.012.8912441
GTCCGGT62750.011.8160881
GTCCCGT70450.011.8064661
GTCCGGA70900.011.5974561
GTCCGTA38300.011.1688011
GTCCGCA73350.010.6268991
GTCGCAA24400.010.5188141
GTCCGAA52600.010.4817811
CGTATTA16450.010.3960193
CTCCGGT118950.010.1492071
CGCTAGT14600.010.0918711
TCCGCTA23550.09.8893572
CTCCGCT143750.09.58855151
GTTCGGT47200.09.566311
GTCCGCG27450.09.5232061
CCCCGAT73600.09.3638191
GTCCGCT82600.09.2066751
GTCCGGG97400.09.1740631
CCCCGCT116100.09.1292581