FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb377.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb377.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28465648
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT51450.015.2426691
GTCCGTT50200.014.9594561
GTCCGTA33400.012.9496311
CCCGTAT38000.012.8829721
CGTATTA16900.011.5245463
GTCCGAA43750.011.407061
GTCCGGA62400.011.3491711
GTTCGTT45000.011.3014381
GTCCGCT70750.011.1517781
TCCGCTA21550.010.8071532
GTCGGAT40550.010.4318561
GTCCGCA62650.010.2417731
CCCCGCT90100.010.1811071
CTCCGCT116250.010.0578371
GTCCCGT59400.010.0019811
GTCCTAT68450.09.65171
CTCGCGT11900.09.5857641
GTTCGGT42100.09.1446141
GTCCAAT108000.09.0657951
GTCCATT181250.08.9420851
Produced by FastQC (version 0.11.5)