FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb376.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb376.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26793012
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT53800.012.7218071
GTCCGTT51300.012.6932181
CCCGTAT35550.011.0970371
GTCCGGA64150.010.4469861
GTCCGCA65550.010.2963711
GTCCGAT70850.09.9286551
GTCCGCT71850.09.7243171
GTCCGAA43250.09.5609651
GTCGCAA19700.09.4095091
GTCCGGG85000.09.2823461
GTCGCGT11807.2759576E-129.2643381
CGATTTA18000.09.23568254
GCCCTAT53800.09.1879711
CTCCGCT117600.09.0937651
GTCGGTT58050.09.0884391
GTTCGTT40600.08.8972571
GTCGGAT41800.08.8692491
GTCCCGT59150.08.8390681
GTCCTAT57350.08.7849851
CGTATTA14150.08.7285523
Produced by FastQC (version 0.11.5)