FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb375.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb375.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28309375
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT49150.016.4389821
GTCCGGT48750.014.2340271
TCCGCTA19000.012.2572212
CCCGTAT35650.012.1319721
GTCCGCG18450.012.1074041
GTCCGCT58100.011.9433531
GTCGCAT29800.011.3237861
CTCCGCT105600.011.2518941
GTCGCGA6101.5932528E-710.9080661
GTCGCAA17250.010.7454611
GTCCGGA60550.010.67515851
GTCCCGT57950.010.2519421
TCGCGAA6051.6853592E-610.2126282
CGCTAGT13250.010.0436531
GTCGGAT39000.09.8712011
GTCCGGG72650.09.8130771
GTCCGAT62450.09.5893161
CCGTATT44050.09.4948112
CCCGCAT54950.09.4277561
GTCCGCA58800.09.3762721
Produced by FastQC (version 0.11.5)