FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb374.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb374.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31942347
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT62150.013.3836651
CGTATTA18500.012.3255853
GTCCGGT57600.011.5527051
CCCGTAT45250.011.0293231
GTCCGTA37600.010.6186571
GTCCGCA76100.010.3056981
GTCCGGG91500.010.2334791
GTCCGGA71050.09.9009251
CTCCGGT116550.09.8691841
GTCCGAA51700.09.8371971
GTCCGCT85850.09.7442981
GTTCGTT49000.09.7002311
CGCTACT25800.09.5799181
CCCGAAT71050.09.2988411
CTCCGCT138950.09.1333661
GTCGGAT53750.09.1082911
GTCCTAT74650.08.9777491
CCGTATT57050.08.9146912
GTCGGTT77250.08.7986431
GTCCCGT71650.08.6239321
Produced by FastQC (version 0.11.5)