FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb373.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb373.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34998127
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT61400.014.011461
CCCGTAT48900.013.6079411
CTCCGCT133400.012.6486951
CGCTAGT15600.012.4920151
GTCCGGT55450.011.6576391
CTCCGGT125500.011.437641
GTCCGGA73350.011.1455521
CCCCGCT115700.010.9685971
CTCCGAT108000.010.9584411
GTCCGAA50500.010.8237971
TCCGCTA23000.010.7460472
CGTATTA21850.010.6531253
CTCCTAT121600.010.1237041
GTCCGCT83450.010.0244261
GTCCCGT74450.010.023241
CTCGTAT85900.09.9045121
CTCCGTT124450.09.8154771
CTCCCGT105800.09.7936381
CCGTATT63600.09.7153722
GTCGCGA6403.4567784E-69.6546521
Produced by FastQC (version 0.11.5)