FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb372.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb372.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28127522
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA14600.013.6655913
GTCCGGT48550.013.0204751
GTCCGTT56800.012.9702331
GTCCGCT78000.010.6027631
CCCGTAT40000.010.5753431
TCCGCTA19300.010.3432352
GTCCGGA66900.010.0174561
CTCCGCT120050.010.0166571
GTCCGAA43050.09.7156971
CGATTTA18700.09.6519724
GTTCGTT42250.09.5621751
GTCCGCA68600.09.4227841
CTCGTAT70100.09.356761
GTCCGGG87850.09.3057441
GTCCATT158200.09.16342451
GTCCTAT60950.09.0458361
CCCCGCT97900.09.0301451
CCCGTCT101200.09.017481
CTCCGGT104400.08.9687151
GTCGCAA20300.08.8971721
Produced by FastQC (version 0.11.5)