FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb371.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb371.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30920760
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT64550.013.6223591
GTCCGGT55350.011.764671
GTCCGGA72850.011.2873861
GTCCGCA75550.010.8210851
GTCGCAT31200.010.6640031
CGCTAGT12550.010.6045221
GTCCGTA41000.010.3177021
CTCCGCT139100.010.1827711
GTCCGCT92250.010.15024951
GTCCGAA50450.09.8924751
CCCGTAT46750.09.7603741
GTCCCGT78050.09.6828021
TCCGCTA21550.09.4841412
CTCCGAT92500.09.4034271
GTCCTAT64350.09.0113131
CGTATTA15300.09.0039693
GTCCATT179200.08.99163151
GTTCCGT69300.08.7105851
CCCCGCT129350.08.6720631
GTCGGAA79800.08.5770211
Produced by FastQC (version 0.11.5)