FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb370.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb370.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31936131
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGGT125550.012.0765751
GTCCGGT55700.012.0318861
CGCTAGT15950.011.9198041
TCCGCTA21050.011.7414332
CTCCGCT143400.011.2362231
GTCCGTT65350.011.2006941
CCCGTAT50350.011.1391571
GTCCGGA73150.011.110961
CCCCGCT117300.010.3731771
CTCCGAT95200.010.28491
GTCCGTA38300.010.1761821
CGTATTA17900.010.0847163
CCCCGAT74600.010.0667221
CCCCTAT54650.09.827841
CCCCGTT73100.09.6230811
CTCCGTT109700.09.4887131
GTCCGAA53100.09.4881421
GTCCGCT89100.09.335341
GTCCCGT78300.09.04470351
CGCTACT26350.09.0190171
Produced by FastQC (version 0.11.5)