FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb369.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb369.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28003392
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT55900.013.4340891
GTCCGGT48600.013.0070331
CCCGTAT44800.011.8823651
CTCCGAT76000.011.4445931
GTCCGGA63900.010.8596271
GTCGCAT26050.010.764831
CTCCGGT104300.010.7545091
GTCCGAA46950.010.7308251
CCCCGCT104150.010.5418211
CTCCGTT95450.010.456981
CTCCGCT125200.010.2120011
CGTATTA14450.010.1910783
CTCCTAT86950.09.9486631
CTCGTAT68550.09.8456351
CCCGAAT64650.09.6308731
GTCCCGT68350.09.5267531
CCCCGAT68700.09.4090351
CGATTTA18300.09.3438314
CGCTAGT13250.09.3265351
GTCGGTT59550.09.2584681
Produced by FastQC (version 0.11.5)