FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb368.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb368.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28213201
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGA68550.012.2727391
GTCCGAA45850.012.2325881
GTCCGGT48150.012.0431271
GTCCGTT60150.011.9321041
CTCCGCT123600.011.1905191
CGTATTA14150.011.0788063
CCCGTAT43750.010.7555561
TCCGCTA19600.010.6701952
CCCCGAT67200.010.18518451
CTCCGGT110250.010.001961
GTCCGTA33400.09.9615581
GTCGGTT61900.09.8286761
CTCGTAT79150.09.7884151
CCCGAAT67700.09.7589221
CCCCGCT96450.09.7575031
GTCCGCA70550.09.4320641
GTCCCGT67750.09.4009391
CCCGTCT104900.09.1074391
GTTCGTT41100.09.0204561
CTCCGTT92150.08.66542151
Produced by FastQC (version 0.11.5)