FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb367.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb367.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28634878
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT54200.012.8903321
CGTATTA16800.012.7246523
CCCGTAT43900.012.6668131
CTCCGCT118450.011.5157671
CGTTCGA32000.011.28203344-45
GTCCGGT49350.011.1716381
CTCCGGT107300.010.7634771
GTCCGCA67300.010.7343211
TCCGCTA21300.010.264182
CTCCGAT81950.010.0332851
GTCCGAA46950.010.0217971
GTCCGGA67250.09.8942241
GTCCCGT63000.09.8827271
CCCCGCT98200.09.7765571
CTCGCGT11750.09.7077681
CTCCGTT93550.09.5512481
CCCGTTT76200.09.5429471
CTCGTAT78850.09.5236051
GTCGGTT65200.09.4763651
GTCCTAT61700.09.3206511
Produced by FastQC (version 0.11.5)