FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb366.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb366.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31711215
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT48000.013.7639431
GTCCGTT66700.012.4704341
CCCGTAT45950.010.8610831
CTCCGCT129800.010.72907451
CTCCTAT92000.010.4359711
CGTATTA17300.010.4344723
CCCGTTT87400.010.0607331
CTCCGGT121550.010.049571
CTCCGTT98650.09.78066351
TAAGTAC140000.09.7383833
GTCCGGA73700.09.7382031
CCCCGAT74000.09.6344941
CTCGTAT89300.09.5273241
GTCCTAT65450.09.5132951
GTCCGTA35700.09.1865021
CTCCGAT101650.09.1646961
GTCCAAT124700.09.1096311
GTTCGTT50900.09.0578121
GTCCCGT77250.09.0445761
GTCCGAA50300.08.976871
Produced by FastQC (version 0.11.5)