FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb365.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb365.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24937898
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGGT93650.013.4499971
GTCCGGT38100.012.6002881
CCCGTAT35650.012.5329251
CTCGCGT9850.012.0639051
GTCCGAA41050.012.0421561
TCCGCTA16450.011.84682752
CCCCGGT49100.011.7135351
CTCCGCT101150.011.6538621
CTCCGAT87300.011.1070831
CGCTAGT11650.011.0159491
CCCCGCT78250.010.8730851
CGTATTA14150.010.7430723
GTCCGTT49850.010.4884581
TAAGTAC119300.010.4326783
CCCGTTT66350.010.31586551
CCCCGAT55750.010.2310561
CCCGAAT61500.010.1246561
CCCCGTT43950.09.949771
CTCCTAT78950.09.8736081
CCCGTCT83100.09.6093131
Produced by FastQC (version 0.11.5)