FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb364.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb364.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32076373
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGTAT44150.013.2418981
GTCCGGT50800.011.9762841
GTCCGAA50900.011.9527551
GTCCGTT68700.011.5540861
CTCGTAT85250.011.2624481
CTCCGGT126050.010.7844751
CCCGAAT80900.010.6929781
GTCCTAT61800.010.613691
CTCCGCT127750.010.4549331
GTCCGCA73200.010.3892631
GTACACT214450.010.3727911
GTCCGTA36200.010.3727611
CCCCGCT113950.09.9274661
CGTATTA16950.09.8090053
CGCTACT25050.09.6769461
GTCCGGA75300.09.6576681
TAAGTAC145000.09.5662433
CGCTAGT15500.09.5061751
TCCGCTA20550.09.4830472
CCCCGTT56300.09.4555211
Produced by FastQC (version 0.11.5)