FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_mb363.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_mb363.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences35309230
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATTA16950.014.0131753
GTCCGGT63150.012.6449121
CCCGTAT52700.012.5367251
GTCCGTT74650.011.2063131
CTCGCGT17700.011.010081
CTCCGGT151000.010.7024161
CTCGTAT101150.010.2910131
CTCCGCT153000.09.8790591
GTCCGGA85150.09.8244421
CCCCGAT86650.09.8189341
GTACACT219200.09.6276941
CCCCGGT80000.09.5062651
TAAGTAC141650.09.4908713
GTTCGTT55300.09.3687421
CGCTAGT16950.09.2538851
CTCCGAT113350.09.1833791
CCCGAAT92700.09.1781091
GTCGTTT101600.09.0758621
GTCCGAA59300.08.9772471
CCCGTTT104850.08.9758721
Produced by FastQC (version 0.11.5)