FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_br4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_br4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29093061
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTT53550.013.9356581
CCCGTAT36300.013.0942531
GTCCGGT46900.012.8711751
GTCGCGA6301.1641532E-1012.8261321
GTCCGGA60550.011.93209651
GTCCGCA64850.011.7272811
GTCCCGT57950.011.3191271
CGTATTA18050.011.3170023
GTCGCAA18350.010.879291
GTCCGAA39800.010.62904551
GTCCGTA35100.010.5626961
GTCGGAT46550.010.3130951
GTCCGCT69550.010.1830171
GTCGCAT26550.010.025611
GTTCGGT47350.09.8376971
GTTCGTT48050.09.7933021
CCGTATT51000.09.6928272
CTCGCGT10405.4569682E-119.5978351
GTCCAAT122700.09.4521931
GTCCTAT69700.09.41095351
Produced by FastQC (version 0.11.5)