FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_br3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_br3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31049770
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT52400.016.0553631
GTCCGTT60850.014.2163761
GTCCGGA68650.012.4626421
GTCCGCT84700.011.5601091
GTTCGTT51300.010.7477791
GTCCGCA70200.010.5624721
GCCGTTA17600.010.5324651
GTTCGGT54400.010.4848081
GTCCCGT70500.010.4501071
GTCCGGG88250.010.394911
GTCCGAA50300.010.3944821
GTCGCAT28100.010.3181451
GTCCAAT126100.010.1771651
CCCGTAT45000.010.1399751
GTCGCAA22200.09.8487931
GTCGTTT98600.09.7376151
TCCGCTA21600.09.6822662
GTCCATT183550.09.5554271
GTCCGAG81500.09.4479061
GTTCCGT69550.09.2943691
Produced by FastQC (version 0.11.5)