FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_br2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_br2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34395872
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT58200.015.43512251
GTCCGGA80250.013.2078191
GTCCGTT71750.012.8514191
GTCCCGT77300.011.9287111
GTCCGTA39950.011.6595171
CCCGTAT47100.011.2014271
GTCCGAA53600.011.0845121
GTCGGAT55750.011.0833171
GTCCGCA77900.010.982631
CGTTCGA41950.010.64443944-45
GTCCGGG101950.010.5830281
GTTCGTT57350.09.77957251
TATGCCG36650.09.72107246-47
CGTATTA20050.09.7139893
GTCCGCT91500.09.5060761
GCGTTCG36850.09.4104944-45
GTCCGAC54100.09.2249361
GTCGGTT81800.09.122581
GTCCTAT75050.09.0564211
CCGTATT62650.09.0281162
Produced by FastQC (version 0.11.5)