FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_br1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_br1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34341872
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGGT63400.012.8948431
GTCCGTT71200.012.7505891
GTCCGAA53200.011.7040611
GTCCGGA78250.011.6018131
GTCCGTA44250.011.6007841
GTCGCGA9900.011.5226641
GTCCGCA80850.010.9935631
GTCCCGT80300.010.2993681
GTTCGTT54950.010.2068381
GTCCTAT75500.010.0727921
GTCCGGG108200.09.9718441
CGTATTA17300.09.8851113
GTCCGCT98500.09.8439821
CCCGTAT52650.09.7499471
GTCGTTT104100.09.7253621
GCCCTAT69700.09.6835021
GTACGGT30300.09.5689451
GTCCGAT97050.09.5502771
GTCGGTT78350.09.5243981
GTCGCAT32950.09.5206231
Produced by FastQC (version 0.11.5)