FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_GG132.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_GG132.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62345313
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC8393691.3463225375097563TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCGCGTATGC3322970.5329943567690485TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTTTGC944870.15155429566934725TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGGATGC760970.122057290818317TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2180050.075.87542
GATCGGA2183900.075.7634051
TCGGAAG2246550.073.361723
CGGAAGA2275450.072.291254
GAGCACA2670400.060.669769
GAAGAGC2786050.058.625296
AGAGCAC2775850.058.54648
AAGAGCA3133350.052.1318247
GGAAGAG3278600.050.1482245
CTCGTAT1065250.042.80110542-43
CGTATGC1377500.042.7793644-45
CGCGTAT424950.042.65314542-43
TATGCCG1392700.042.1419246-47
ACGGTAG1941000.041.96507632-33
TCGCGTA426150.041.85385540-41
TCGTATG1019700.041.8343342-43
ATCGCGT497950.041.64721740-41
TGCCGTC1469400.041.49558348-49
ATGCCGT1327200.041.46388646-47
TCTCGTA1083300.041.4200140-41
Produced by FastQC (version 0.11.5)