FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_GG131.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_GG131.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64843675
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC7033541.0846917606073374TruSeq Adapter, Index 7 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCGCGTATGC3227570.4977463106463352TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1245300.042.07346344-45
TATGCCG1256350.041.80361646-47
CGCGTAT408500.041.74310742-43
TGCCGTC1288100.041.3872448-49
CTCGTAT893050.041.3237542-43
CCGTCTT1156750.041.24252350-51
ATGCCGT1225050.041.16163646-47
TCGCGTA409250.040.7391340-41
ATCGCGT443850.040.46887640-41
TCGTATG869250.040.4634442-43
GCCGTCT1178600.039.7650148-49
TCTCGTA907300.039.5920340-41
GATCGCG479600.039.2013938-39
CGTCTTC1134750.039.12028550-51
ATCTCGT991500.039.00362840-41
GATCTCG1037650.038.05957838-39
GCGTATG440400.037.39288342-43
GGATCGC516450.037.3838238-39
GTATGCC1383750.037.00216744-45
CCGTCGT138350.036.10187550-51
Produced by FastQC (version 0.11.5)