FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_GG130.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_GG130.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64674995
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC6748651.043471282835043TruSeq Adapter, Index 22 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCGCGTATGC2267130.3505419675718568TruSeq Adapter, Index 22 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC1070400.041.8852644-45
CTCGTAT857550.041.66625242-43
CGCGTAT286650.041.54996542-43
TATGCCG1077300.041.4450246-47
ATGCCGT1046600.041.36034846-47
TCGTATG822400.041.3332242-43
TCGCGTA287650.041.22474340-41
TGCCGTC1121150.041.09521548-49
TCTCGTA875550.040.68573440-41
TACGATC1405450.040.65944736-37
CCGTCTT995700.040.4188650-51
ATCGCGT322950.040.2633240-41
CGTACGA1426700.040.1154434-35
CGATCTC1061950.039.95265638-39
GCCGTCT986100.039.8369748-49
ATCTCGT987800.039.63035640-41
CGATCGC367950.039.62596538-39
GTACGAT1420550.039.57184234-35
GATCTCG1061500.039.37890638-39
ACGATCG368050.039.32573736-37
Produced by FastQC (version 0.11.5)