FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_GG129.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_GG129.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58492437
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC13164622.250653362245789TruSeq Adapter, Index 21 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCGCGTATGC5474830.9359893826957492TruSeq Adapter, Index 21 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGAAGA3484100.056.2106064
TCGGAAG3517350.055.767133
ATCGGAA3525850.055.7287522
GATCGGA3579000.054.9277151
GAGCACA3568050.054.18029
AGAGCAC3689900.052.5171788
GAAGAGC3796000.051.2620126
AAGAGCA4052350.048.070777
GGAAGAG4251000.045.9640855
CGTATGC2110000.044.40603644-45
CGCGTAT632900.044.39912842-43
TATGCCG2120350.044.2744146-47
CTCGTAT1505150.044.21195642-43
ATCGCGT643900.043.93297640-41
TGCCGTC2125300.043.84160648-49
TTCGATC2199200.043.76843336-37
TCGCGTA632650.043.75317840-41
ATGCCGT2101100.043.69881446-47
CCGTCTT2035250.043.6499450-51
TCGTATG1494050.043.60256642-43
Produced by FastQC (version 0.11.5)