FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_GG128.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_GG128.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62547735
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC3945400.6307822337611426TruSeq Adapter, Index 20 (98% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCGCGTATGC1667670.26662356358707473TruSeq Adapter, Index 20 (97% over 42bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG740400.038.54015446-47
CGTATGC745750.038.20952244-45
ATGCCGT733050.037.724546-47
TGCCGTC759400.037.36323548-49
CCGTCTT735850.037.2626150-51
CGCGTAT234900.037.22648242-43
CTCGTAT543950.036.7493442-43
TCGTATG533200.036.46135742-43
TCGCGTA236400.036.06685340-41
GCCGTCT754300.035.83991648-49
TCTCGTA556000.035.19765540-41
CGTCTTC752350.035.13215650-51
ATCGCGT251200.034.90626540-41
ATCTCGT606550.033.22750540-41
GTATGCC871400.032.1357544-45
GCGTATG270000.031.48984542-43
CATCGCG279850.030.91751138-39
CATCTCG660800.030.11569238-39
GCTTGAA881250.028.80765758-59
TGCTTGA912850.026.8087458-59
Produced by FastQC (version 0.11.5)