FastQCFastQC Report
Thu 12 Nov 2020
HVJ7MDRXX_n01_GG127.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ7MDRXX_n01_GG127.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59193385
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC3374840.5701380314709151TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCTCGTATGC1987130.33570136257624056TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTGGTATGC1298200.21931504677422994TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCGCGTATGC1264820.21367590314356916TruSeq Adapter, Index 3 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCTGGTATGC673790.11382859757048866TruSeq Adapter, Index 20 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCGAGATCGCGTATGC622370.1051418160998902TruSeq Adapter, Index 20 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA1836100.070.2955862
GATCGGA1854450.069.6410141
TCGGAAG1927700.066.802653
CGGAAGA1952700.065.8703164
GAGCACA2292800.055.3768469
GAAGAGC2459350.052.0612146
AGAGCAC2447850.051.9895138
AAGAGCA2781250.046.0561837
GGAAGAG2958650.043.551525
TATGCCG1190850.042.29347246-47
CTCGTAT672300.042.12574442-43
ACGTCGA531250.041.98931532-33
CGTATGC880300.041.79389644-45
CGCGTAT243900.041.59793542-43
TCGTATG653100.041.34232342-43
ATGCCGT1169550.041.17302346-47
TCGCGTA243350.041.06813440-41
TCTCGTA686050.040.71103340-41
TGCCGTC1247500.040.55566848-49
CGTCGAG539800.040.4222532-33
Produced by FastQC (version 0.11.5)