FastQCFastQC Report
Thu 29 Feb 2024
HVJ5VDRX3_4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ5VDRX3_4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031824387
Sequences flagged as poor quality0
Sequence length90
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACATGGGGGGCGGTCGTGCATTCCGCA46285260.4485769146683291Clontech SMARTer II A Oligonucleotide (100% over 25bp)
AAGCAGTGGTATCAACGCAGAGTACATGGGGGCGGTCGAGTGCCTCACAG13435810.13021411559252088Clontech SMARTer II A Oligonucleotide (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11768880.11405894402455134No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)