FastQCFastQC Report
Thu 29 Feb 2024
HVJ5VDRX3_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ5VDRX3_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1031824387
Sequences flagged as poor quality0
Sequence length10
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTTATCA30254838829.321693866884736No Hit
AATCTCACGC27776196426.919499819885534No Hit
GTGTACACTA22398378121.70754867029519No Hit
ATGTTCATAG18622105218.047746723784304No Hit
AGTTCTCGGT140891641.3654614271100767No Hit
AGATCTCGGT82526870.7998150755085807Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG18027610.17471587439811112No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)