FastQCFastQC Report
Fri 17 Dec 2021
HVJ55BGXK_n01_pIXHALO_ampPt-B_v.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ55BGXK_n01_pIXHALO_ampPt-B_v.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23779644
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT3240521.362728558930487TruSeq Adapter, Index 2 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTTT487380.20495681095982765TruSeq Adapter, Index 2 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTATGCC306140.12874036297599745TruSeq Adapter, Index 2 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)