FastQCFastQC Report
Fri 17 Dec 2021
HVJ55BGXK_n01_AtTGA5_ZmLeaf-B_v.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ55BGXK_n01_AtTGA5_ZmLeaf-B_v.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33613331
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTAT610340.18157676786034685TruSeq Adapter, Index 7 (97% over 38bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCATCTCGTATGCC362840.10794526731075835TruSeq Adapter, Index 7 (97% over 35bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)