FastQCFastQC Report
Fri 26 Jan 2024
HVJ2YDRX3_n01_R23_macrophage.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVJ2YDRX3_n01_R23_macrophage.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55632030
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAAC1521845427.355561175819037No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAA1473468226.485968604776783No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT1101382219.797627374014574No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACACCGTACCCCTACCAAC27103024.871837321054076No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAA26273104.722657073631862No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACACCGTACCCCTACCAACT19534523.5113800449129755No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAAC4884360.8779762305995306No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGAACCCCTACCAAC3593240.6458941009342999No Hit
ATCGATTCCTTGGTTCTTGAGGAAAGGACGAAACACCGAACCCCTACCAA3060570.5501453029846295No Hit
CGATCATGATCGTCTTGTGCAAAGGACGAAACACCGAACCCCTACCAACT1791670.3220572752782884No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAACTCCGAACCCCTACCAA1417150.25473634523133526No Hit
ACGATCGATAGGTAAGGTCTTGTGGAAAGGACGAAACACCGAACCCCTAC1402240.25205623451094633No Hit
CGATCGATACAGGTATTCTTGTGGAAAGGACGAAACACCGAACCCCTACC1160950.20868373848662364No Hit
ATCGATTCCTTGGTTCTTGTGGAAAGGACGAAAGACCGAACCCCTACCAA1034210.18590189860050046No Hit
CGATCATGATCGTCTTGTGGAAAGGACGAAACAGCGAACCCCTACCAACT994960.1788466104868005No Hit
GATCGATAACGCATTTCTTGTGGAAAGGACGAAACACCGAACCCCTACCA842560.1514523198236699No Hit
TCGATCGTTACCATCTTGTGGAAAGGACGAAACTCCGTACCCCTACCAAC770170.1384400317586829No Hit
TCGATCGTTACCATCTGTGGAAAGGACGAAACACCGAACCCCTACCAACT759970.13660655561193794No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACACCGTACCCCTACCAAC641550.11532025705335577No Hit
TCGATCGTTACATCTTGTGGAAAGGACGAAACACCGAACCCCTACCAACT635030.11414827033994625No Hit
ATCGATTCCTTGGTTCTTGTGGAAGGACGAAACACCGAACCCCTACCAAC611530.1099240850998966No Hit
TCGATCGTTACCATCTTGTGGAAGGACGAAACACCGAACCCCTACCAACT562460.10110362681354608No Hit
TCGATCGTTACCATCTTGTCGAAAGGACGAAACTCCGAACCCCTACCAAC556870.10009880998410449No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)